BioNovoGene.Analytical.MassSpectrometry.Math.ActivationMethods

ActivationMethods {BioNovoGene.Analytical.MassSpectrometry.Math}

.NET clr documentation

ActivationMethods

Description

Activation Types enum

This enum mirrors ThermoFisher.CommonCore.Data.FilterEnums.ActivationType/CollisionType 在质谱数据中，Collision Type（碰撞类型）和Activation Method（活化方法）通常是指代同一概念的不同术语。在质谱分析中，为了将大分子（如蛋白质、多肽、聚合物等）打碎成更小的片段以进行分析，常使用一种称为碰撞诱导解离（Collision-Induced Dissociation, CID）的技术。这个过程中，使用高能碰撞来激活分子，从而产生碎片。活化方法（Activation Method）是一个更广泛的术语，它包括了碰撞诱导解离（CID）以及其他可能的活化技术，例如电子捕获解离（Electron Capture Dissociation, ECD）、红外多光子解离（Infrared Multiphoton Dissociation, IRMPD）和黑体红外辐射解离（Blackbody Infrared Radiative Dissociation, BIRD）等。碰撞类型（Collision Type）则更具体地指明了在碰撞诱导解离过程中所使用的特定类型，例如使用氩、氖等气体作为碰撞气体来产生碰撞。因此，虽然这两个术语在语境上有所不同，但它们都是描述在质谱分析中用来使分子离子发生裂解的方法。在具体的实验和文献中，它们的含义可能会根据上下文有所变化，但基本上可以认为是相关的概念。

除了碰撞诱导解离（CID），还有其他几种常见的活化方法，用于在质谱分析中产生离子碎片，以便进行结构解析和表征。这些方法包括： + 电子捕获解离（Electron Capture Dissociation, ECD）：这种方法在低温下使用电子来断裂分子中的键，特别适用于大分子，如蛋白质和肽。ECD通常用于保持分子中的非共价相互作用，以便更好地理解分子结构。 + 红外多光子解离（Infrared Multiphoton Dissociation, IRMPD）：IRMPD使用红外激光的光子能量来激发分子振动，从而导致分子内部的键断裂。这种方法对于具有强红外吸收的分子特别有效。 + 黑体红外辐射解离（Blackbody Infrared Radiative Dissociation, BIRD）：BIRD是一种利用黑体辐射源的红外光子能量来解离分子中的键的方法。这种方法通常用于研究大分子和生物大分子。 + 快速加热解离（Pulsed Q-TOF）:这种方法通过在离子飞行管中施加快速电压脉冲，使离子加速并获得足够的动能，从而在碰撞过程中发生解离。 + 紫外光解离（Ultraviolet Photodissociation, UVPD）：UVPD使用紫外光来激发离子，导致特定的键断裂。这种方法对于具有特定紫外线吸收特性的分子非常有效。 + 活化离子飞行时间质谱（Activating Ion Time-of-Flight, ACTOF）：这是一种将离子在飞行管中加速并使其在特定的距离内发生碰撞，从而产生碎片的方法。 + 溶剂辅助解离（Solvent Assisted Dissociation, SAD）：SAD是一种在离子源中引入溶剂，以促进离子在形成过程中的解离。这些活化方法的选择取决于分析物的类型、所需的解析水平和质谱仪的配置。不同的活化方法可以提供不同的碎片模式和结构信息，因此在实际应用中，研究人员会根据具体的研究目的选择最合适的活化方法。 (这个枚举值为Byte类型，请勿修改，否则mzPack文件格式读写会出现问题)

Declare

            
# namespace BioNovoGene.Analytical.MassSpectrometry.Math
export class ActivationMethods extends Enum {
   # Collision-Induced Dissociation
   #  
   #  In collision-induced dissociation (CID), activation of the selected ions 
   #  occurs by collision(s) with neutral gas molecules in a collision cell. 
   #  This experiment can be done at high (keV) collision energies, using tandem 
   #  sector and time-of-flight instruments, or at low (eV range) energies, in 
   #  tandem quadrupole and ion trapping instruments.
   CID: ActivationMethods = 0;

   # Multi Photon Dissociation
   MPD: ActivationMethods = 1;

   # Electron Capture Dissociation
   #  
   #  unique fragmentation mechanisms of multiply-charged species can be studied 
   #  by electron-capture dissociation (ECD). The ECD technique has been recognized 
   #  as an efficient means to study non-covalent interactions and to gain 
   #  sequence information in proteomics applications.
   ECD: ActivationMethods = 2;

   # Pulsed Q Dissociation
   PQD: ActivationMethods = 3;

   # Electron Transfer Dissociation
   #  
   #  Electron transfer dissociation (ETD) involves transfer of an electron from a 
   #  radical anion to the analyte cation and also produces c and z type ions, and 
   #  has been implemented with wide variety of mass analyzers, most commonly ion 
   #  traps.
   ETD: ActivationMethods = 4;

   # High-energy Collision-induce Dissociation (psi-ms: beam-type collision-induced dissociation)
   HCD: ActivationMethods = 5;

   # Any activation type
   AnyType: ActivationMethods = 6;

   # Supplemental Activation
   SA: ActivationMethods = 7;

   # Proton Transfer Reaction
   PTR: ActivationMethods = 8;

   # Negative Electron Transfer Dissociation
   NETD: ActivationMethods = 9;

   # Negative Proton Transfer Reaction
   NPTR: ActivationMethods = 10;

   # Ultraviolet Photo Dissociation
   UVPD: ActivationMethods = 11;

   # Mode A
   ModeA: ActivationMethods = 12;

   # Mode B
   ModeB: ActivationMethods = 13;

   # Mode C
   ModeC: ActivationMethods = 14;

   # Mode D
   ModeD: ActivationMethods = 15;

   # Mode E
   ModeE: ActivationMethods = 16;

   # Mode F
   ModeF: ActivationMethods = 17;

   # Mode G
   ModeG: ActivationMethods = 18;

   # Mode H
   ModeH: ActivationMethods = 19;

   # Mode I
   ModeI: ActivationMethods = 20;

   # Mode J
   ModeJ: ActivationMethods = 21;

   # Mode K
   ModeK: ActivationMethods = 22;

   # Mode L
   ModeL: ActivationMethods = 23;

   # Mode M
   ModeM: ActivationMethods = 24;

   # Mode N
   ModeN: ActivationMethods = 25;

   # Mode O
   ModeO: ActivationMethods = 26;

   # Mode P
   ModeP: ActivationMethods = 27;

   # Mode Q
   ModeQ: ActivationMethods = 28;

   # Mode R
   ModeR: ActivationMethods = 29;

   # Mode S
   ModeS: ActivationMethods = 30;

   # Mode T
   ModeT: ActivationMethods = 31;

   # Mode U
   ModeU: ActivationMethods = 32;

   # Mode V
   ModeV: ActivationMethods = 33;

   # Mode W
   ModeW: ActivationMethods = 34;

   # Mode X
   ModeX: ActivationMethods = 35;

   # Mode Y
   ModeY: ActivationMethods = 36;

   # Mode Z
   ModeZ: ActivationMethods = 37;

   # Last Activation
   LastActivation: ActivationMethods = 38;

   # Collisional activation upon impact of precursor ions on solid surfaces, 
   #  surface-induced dissociation (SID), is gaining importance as an alternative 
   #  to gas targets and has been implemented in several different types of mass 
   #  spectrometers.
   SID: ActivationMethods = 39;

   # Trapping instruments, such as quadrupole ion traps and Fourier transform ion 
   #  cyclotron resonance instruments, are particularly useful for the photoactivation 
   #  of ions, specifically for fragmentation of precursor ions by infrared 
   #  multiphoton dissociation (IRMPD). IRMPD is a non-selective activation method 
   #  and usually yields rich fragmentation spectra.
   IRMPD: ActivationMethods = 40;

   EIEIO: ActivationMethods = 41;

   HotECD: ActivationMethods = 42;

   EID: ActivationMethods = 43;

   OAD: ActivationMethods = 44;

   # Unknown activation type
   Unknown: ActivationMethods = 255;

}

.NET clr type reference tree

this class extends from Enum class: Enum
use by field member CID: ActivationMethods
use by field member MPD: ActivationMethods
use by field member ECD: ActivationMethods
use by field member PQD: ActivationMethods
use by field member ETD: ActivationMethods
use by field member HCD: ActivationMethods
use by field member AnyType: ActivationMethods
use by field member SA: ActivationMethods
use by field member PTR: ActivationMethods
use by field member NETD: ActivationMethods
use by field member NPTR: ActivationMethods
use by field member UVPD: ActivationMethods
use by field member ModeA: ActivationMethods
use by field member ModeB: ActivationMethods
use by field member ModeC: ActivationMethods
use by field member ModeD: ActivationMethods
use by field member ModeE: ActivationMethods
use by field member ModeF: ActivationMethods
use by field member ModeG: ActivationMethods
use by field member ModeH: ActivationMethods
use by field member ModeI: ActivationMethods
use by field member ModeJ: ActivationMethods
use by field member ModeK: ActivationMethods
use by field member ModeL: ActivationMethods
use by field member ModeM: ActivationMethods
use by field member ModeN: ActivationMethods
use by field member ModeO: ActivationMethods
use by field member ModeP: ActivationMethods
use by field member ModeQ: ActivationMethods
use by field member ModeR: ActivationMethods
use by field member ModeS: ActivationMethods
use by field member ModeT: ActivationMethods
use by field member ModeU: ActivationMethods
use by field member ModeV: ActivationMethods
use by field member ModeW: ActivationMethods
use by field member ModeX: ActivationMethods
use by field member ModeY: ActivationMethods
use by field member ModeZ: ActivationMethods
use by field member LastActivation: ActivationMethods
use by field member SID: ActivationMethods
use by field member IRMPD: ActivationMethods
use by field member EIEIO: ActivationMethods
use by field member HotECD: ActivationMethods
use by field member EID: ActivationMethods
use by field member OAD: ActivationMethods
use by field member Unknown: ActivationMethods

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